Laboratory teaching fundamental techniques in biochemistry. $40 lab fee. Cross-listed as CHEM 420L. Students majoring in Biology or Chemistry must earn a grade of C or higher. Prerequisites: BIOL 110 and CHEM 310.
Prerequisite(s) / Corequisite(s):
BIOL 110 and CHEM 310.
Course Rotation for Day Program:
Most current editions of the following:
Experimental procedures should be taken from texts such as:
Fundamental Laboratory Approaches for Biochemistry and Biotechnology
By Ninfa & Ballou (John Wiley and Sons, Inc.) Recommended
Experiments in Biochemistry: A Hands-On Approach
By S.L. Farrell & L.W. Taylor (Thompson Brooks/Cole) Recommended
Modern Experimental Biochemistry
By Boyer (Benjamin Cummings) Recommended
Writing in Biology
By Karin Knisely (MacMillan) Recommended
Course Learning Outcomes
Demonstrate facility with laboratory equipment and techniques used in modern experimental biochemistry.
Employ the scientific method to design and conduct experiments.
Utilize critical analysis skills to interpret data and draw conclusions.
Use scientific writing in laboratory reports.
Perform and evaluate experiments on the isolation, purification, and analysis of macromolecules.
Major Topics/Skills to be Covered:
Basic laboratory practices including safety and utilization of equipment including centrifuges, spectrophotometers, electrophoresis equipment, and liquid chromatography.
Calculating the capacity of buffers and determining their effective use in experiments.
Characterization of biomolecules utilizing column chromatography (affinity, gel-filtration, ion-exchange) and electrophoresis (isoelectric focusing).
Determination of protein concentration.
Isolation, purification, and characterization (kinetics) of enzymes.
Performing Western Blot analysis.
Writing laboratory reports based on the format used in scientific journals.
Recommended maximum class size for this course: 18
NOTE: The intention of this master course syllabus is to provide an outline of the contents of this course, as specified by
the faculty of Columbia College, regardless of who teaches the course, when it is taught, or where it is taught. Faculty members teaching this
course for Columbia College are expected to facilitate learning pursuant to the course learning outcomes and cover the subjects listed in the Major Topics/Skills to be Covered section.
However, instructors are also encouraged to cover additional topics of interest so long as those topics are relevant to the course's
subject. The master syllabus is, therefore, prescriptive in nature but also allows for a diversity of individual approaches to course material.