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Master Syllabus

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Administrative Unit: Physical and Biological Sciences Department
Course Prefix and Number: BIOL 410L
Course Title: Methods in Molecular Biotechnology
Number of:
Credit Hours 2
Lecture Hours 0
Lab Hours 2
Catalog Description: Laboratory experiences to complement BIOL 410. $30 lab fee. Prerequisite: BIOL 342L with a grade of C or higher. Concurrent enrollment in BIOL 410 is required.
Prerequisite(s) / Corequisite(s): BIOL 342L with a grade of C or higher. Concurrent enrollment in BIOL 410 is required.
Course Rotation for Day Program:

Offered Spring.

Text(s): Most current editions of the following:

Experimental procedures may be taken from texts such as the following:

Exercises for the Molecular Biology Laboratory
By P. Giufoile (Morton Publishing)
Unraveling DNA: Molecular Biology for the Laboratory
By M.R. Winfrey, M.A. Rott, & A.T. Wortman (Prentice Hall)
Biotechnology Explorations: Applying the Fundamentals
By J.A. Scheppler, P.E. Cassin, & R.M. Gambier (ASM Press)
Course Objectives
  • To perform broadly applicable lab techniques in molecular biology.
    Measurable Learning
  • Effectively employ laboratory equipment used in molecular biology techniques.
  • Use the scientific method to design and conduct experiments.
  • Perform and evaluate experiments in the manipulation and analysis of DNA, RNA and proteins.
  • Use critical analysis skills to interpret data and draw conclusions.
  • Write lab reports.
    Topical Outline: This course will address the following topics, time and equipment permitting:

  • Basic lab techniques and equipment use
  • Methods in DNA isolation and analysis such as restriction enzyme digests, electrophoresis, restriction fragment length polymorphism (RFLP) analysis, Southern blotting, DNA probe preparation, hybridization and detection
  • Polymerase chain reaction
  • Methods in RNA expression, isolation, and analysis, such as in vitro transcription and Northern blotting
  • DNA cloning: preparation of competent cells, plasmid DNA preparation, generating, isolating and analyzing recombinant clones
  • Methods in protein isolation and analysis, such as in vitro translation, fractionation, electrophoretic separation, Western blot analysis

    Recommended maximum class size for this course: 16

    Library Resources:

    Online databases are available at You may access them from off-campus using your CougarTrack login and password when prompted.

    Prepared by: Frank Somer Date: April 7, 2015
    NOTE: The intention of this master course syllabus is to provide an outline of the contents of this course, as specified by the faculty of Columbia College, regardless of who teaches the course, when it is taught, or where it is taught. Faculty members teaching this course for Columbia College are expected to facilitate learning pursuant to the course objectives and cover the subjects listed in the topical outline. However, instructors are also encouraged to cover additional topics of interest so long as those topics are relevant to the course's subject. The master syllabus is, therefore, prescriptive in nature but also allows for a diversity of individual approaches to course material.

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