Laboratory experiences to complement BIOL 410. $30 lab fee. Prerequisite: BIOL 342L with a grade of C or higher. Concurrent enrollment in BIOL 410 is required.
Prerequisite(s) / Corequisite(s):
BIOL 342L with a grade of C or higher. Concurrent enrollment in BIOL 410 is required.
Course Rotation for Day Program:
Most current editions of the following:
Experimental procedures may be taken from texts such as the following:
Exercises for the Molecular Biology Laboratory
By P. Giufoile (Morton Publishing) Recommended
Unraveling DNA: Molecular Biology for the Laboratory
By M.R. Winfrey, M.A. Rott, & A.T. Wortman (Prentice Hall) Recommended
Biotechnology Explorations: Applying the Fundamentals
By J.A. Scheppler, P.E. Cassin, & R.M. Gambier (ASM Press) Recommended
To perform broadly applicable lab techniques in molecular biology.
Effectively employ laboratory equipment used in molecular biology techniques.
Use the scientific method to design and conduct experiments.
Perform and evaluate experiments in the manipulation and analysis of DNA, RNA and proteins.
Use critical analysis skills to interpret data and draw conclusions.
Write lab reports.
This course will address the following topics, time and equipment permitting:
Basic lab techniques and equipment use
Methods in DNA isolation and analysis such as restriction enzyme digests, electrophoresis, restriction fragment length polymorphism (RFLP) analysis, Southern blotting, DNA probe preparation, hybridization and detection
Polymerase chain reaction
Methods in RNA expression, isolation, and analysis, such as in vitro transcription and Northern blotting
DNA cloning: preparation of competent cells, plasmid DNA preparation, generating, isolating and analyzing recombinant clones
Methods in protein isolation and analysis, such as in vitro translation, fractionation, electrophoretic separation, Western blot analysis
Recommended maximum class size for this course: 16
NOTE: The intention of this master course syllabus is to provide an outline of the contents of this course, as specified by
the faculty of Columbia College, regardless of who teaches the course, when it is taught, or where it is taught. Faculty members teaching this
course for Columbia College are expected to facilitate learning pursuant to the course objectives and cover the subjects listed in the topical
outline. However, instructors are also encouraged to cover additional topics of interest so long as those topics are relevant to the course's
subject. The master syllabus is, therefore, prescriptive in nature but also allows for a diversity of individual approaches to course material.