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Master Syllabus

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Administrative Unit: Physical and Biological Sciences Department
Course Prefix and Number: BIOL 410
Course Title: Molecular Biotechnology
Number of:
Credit Hours 3
Lecture Hours 3
Lab Hours 0
Catalog Description:

Methodology and applications in molecular biology, with special emphasis on biotechnology. Prerequisite: BIOLĀ 254 with a grade of C or higher. Concurrent enrollment in BIOL 410L is strongly recommended.

Prerequisite(s) / Corequisite(s):

BIOLĀ 254 with a grade of C or higher. Concurrent enrollment in BIOL 410L is strongly recommended.

Course Rotation for Day Program:

Offered Spring.

Text(s): Most current editions of the following:

Molecular biotechnology books designed to introduce a wide range of applications; text should be supplemented with current peer-reviewed journal articles.

By J.E. Smith (Cambridge University Press)
From Genes to Genomes: Concepts and Applications of DNA Technology
By J.W. Dale & M. vonSchantz (Wiley & Sons)
An Introduction to Biotechnology
By W.J. Thieman & M.A. Palladino (Pearson Education)
Course Objectives
  • To examine basic techniques used in molecular biotechnology.
  • To apply molecular and biotechnology techniques to experimental situations.
  • To assess the benefits and hazards of molecular biotechnology.
    Measurable Learning
  • Describe the different techniques used in recombinant DNA technology.
  • Determine which technique(s) is appropriate in solving a specific experimental problem.
  • Delineate the steps used in gene manipulation, gene expression techniques, and recombinant protein development, and relate these processes to current commercial and research applications.
  • Describe the applications of molecular biology in areas such as transgenic plants and animals, vaccines and therapeutic agents.
  • Assess the realized and/or potential benefits and risks associated with molecular biotechnology.
  • Explain the processes used to sequence entire genomes, and give examples of the uses and implications of the Human Genome Project.
    Topical Outline:
  • History of molecular biotechnology
  • Recombinant DNA technology
  • Manipulation of gene expression in prokaryotes
  • Recombinant protein production in eukaryotic cells
  • Production of therapeutic agents, vaccines, commercial products by recombinant microorganisms
  • Bioremediation
  • Microbial insecticides
  • Methodology and applications of genetic engineering of plants
  • Transgenic animals
  • Human molecular genetics, gene therapy
  • Legal and patent issues in biotechnology

    Recommended maximum class size for this course: 20

    Library Resources:

    Online databases are available at the Columbia College Stafford Library.  You may access them using your CougarTrack login and password when prompted.

    Prepared by: Alan James Date: January 18, 2015
    NOTE: The intention of this master course syllabus is to provide an outline of the contents of this course, as specified by the faculty of Columbia College, regardless of who teaches the course, when it is taught, or where it is taught. Faculty members teaching this course for Columbia College are expected to facilitate learning pursuant to the course objectives and cover the subjects listed in the topical outline. However, instructors are also encouraged to cover additional topics of interest so long as those topics are relevant to the course's subject. The master syllabus is, therefore, prescriptive in nature but also allows for a diversity of individual approaches to course material.

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