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Master Syllabus

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Administrative Unit: Physical and Biological Sciences Department
Course Prefix and Number: BIOL 290L
Course Title: Principles of Cell Biology Laboratory
Number of:
Credit Hours 2
Lecture Hours 0
Lab Hours 2
Catalog Description:

Laboratory experiences to complement BIOL 290. Students majoring in Biology must earn a grade of C or higher. $40 lab fee. Prerequisites: BIOL 110L; BIOL 290 or concurrent enrollment.

Prerequisite(s) / Corequisite(s): BIOL 110L; BIOL 290 concurrent enrollment.
Course Rotation for Day Program: Offered Fall.
Text(s): Most current editions of the following:

Current Protocols in Cell Biology
By Bonifacino, et. al. (Wiley)
Category/Comments - CD-ROM
Molecular Biology: A Lab Manual
By Sambrook et. al. (Cold Spring Harbor Press)
Cell Biology: A Laboratory Handbook
By Celis (Academic Press)
Cell Biology: Essential Techniques
By Rickwood and Harris (Wiley)
Writing in Biology
By Karin Knisely (MacMillan)
Course Learning Outcomes
  1. Demonstrate facility with a variety of current laboratory techniques.
  2. Perform all techniques using basic laboratory safety procedures.
  3. Interpret data and draw conclusions using critical analytical skills.
  4. Write laboratory reports based on the format followed in scientific journals.
Major Topics/Skills to be Covered:
  • Perform all techniques using basic laboratory safety procedures.
  • Demonstrate proper use of equipment including micropipettors, spectrophotometers, centrifuges, electrophoresis equipment, thermocyclers, and cell transformation machines.
  • Perform subcellular fractionation and successfully isolate different types of subcellular organelles confirming their purity via specific cellular proteins.
  • Use a cell counting chamber to determine the number of cells in different samples.
  • Calculate serial dilutions of cells.
  • Use microscopes to determine cell types, presence and localization of particular subcellular organelles and/or biological molecules.
  • Analyze the cell cycle.
  • Interpret data and draw conclusions using critical analytical skills.
  • Write lab reports based on the format followed in scientific journals.
Culminating Experience Statement:

Material from this course may be tested on the Major Field Test (MFT) administered during the Culminating Experience course for the degree. The results of the tests are used by faculty to improve the general education curriculum at the College.


Recommended maximum class size for this course: 18

Library Resources:

Online databases are available at You may access them from off-campus using your CougarTrack login and password when prompted.

Prepared by: Kent Strodtman Date: April 10, 2015
NOTE: The intention of this master course syllabus is to provide an outline of the contents of this course, as specified by the faculty of Columbia College, regardless of who teaches the course, when it is taught, or where it is taught. Faculty members teaching this course for Columbia College are expected to facilitate learning pursuant to the course learning outcomes and cover the subjects listed in the Major Topics/Skills to be Covered section. However, instructors are also encouraged to cover additional topics of interest so long as those topics are relevant to the course's subject. The master syllabus is, therefore, prescriptive in nature but also allows for a diversity of individual approaches to course material.

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